Extended Data Fig. 11: Data processing scheme of the 2D crystallography experiment.
From: The conserved HIV-1 spacer peptide 2 triggers matrix lattice maturation
a, 150 micrographs were collected randomly from each grid. Six grids were prepared simultaneously, three grids of the myrMA -SP2 control group and three grids of the myrMA +SP2 experimental group. b, Particles were picked randomly as a grid of points and extracted in cryoSPARC. c, The extracted particles were subjected to an initial round of 2D classification. 2D classes showing any kind of MA lattice were selected and subjected to a second round of 2D classification. d, Classes with mature and immature lattices were selected and particles belonging to these classes were used in the final statistical analysis.
