Fig. 1: Heterogeneity and stability of neuronal activity patterns in relation to spontaneous movements.
From: Brain-wide presynaptic networks of functionally distinct cortical neurons
a, Models of input connectivity for functionally distinct PNs (triangles of different colours). NA, noradrenaline. b, Experimental paradigm. IR, infrared. c, Example time course of whisker movements (WM) and locomotion speed (LS). Cyan-shaded, Wonly; magenta-shaded, WL. d, Fraction of time spent per behavioural event type (n = 5 sessions, 5 mice). NM, no movement. e, Whisker movement duration versus amplitude for Wonly (n = 1,268) and WL (n = 321). f,g, Prediction of whisker movements and locomotion speed from population activity. R2, variance explained. f, Example session. Top, raster plot of neuronal activity; neurons (n = 201) are sorted from top to bottom by decreasing weight on the first principal component. Bottom, whisker movements (cyan) and locomotion speed (magenta) overlaid with predicted (grey) traces (P < 0.0001 for both; r, correlation). g, Independent sessions (n = 6 FOVs, 5 mice; WM, P = 0.00011; LS, P = 0.0010; one-sided paired-sample t-test). h, Fraction of movement-uncorrelated (n = 715), Movdown (n = 123) and Movup (n = 286) neurons. i, Peri-event time histogram (PETH) of neuronal activity (n as in h). WL events are time-normalized from onset to offset (vertical bars). Norm., normalized. j, Example imaging FOV (top) and activity of example Movup neurons during spontaneous movements at days 1 and 3 (bottom). Black, F; grey, deconvolved F; normalized to maximum. Scale bar, 100 µm. k, Correlation (r) between the activity of individual neurons and whisker movements at days 3–4 versus day 1 (n = 682, 6 FOVs, 5 mice; P < 0.0001, regression). l, Prediction of whisker movements across days. Left, decoder scheme. Middle, variance explained across days (P < 0.0001, one-sided paired-sample t-test). Right, out-of-sample R2 ratio for day 3 versus day 1 (n = 3 FOVs, 3 mice) and day 4 versus day 1 (n = 3 FOVs, 2 mice). g,l, In box plots, the central line and box represent the median and 25th–75th percentiles, and whiskers extend to the most extreme data points excluding outliers (larger than 1.5× the interquartile range).
