Extended Data Fig. 8: Absence of presynaptic labelling in sham single-cell-based monosynaptic input tracing experiments.
From: Brain-wide presynaptic networks of functionally distinct cortical neurons
a, Imaging FOV for an example single-cell-based monosynaptic retrograde tracing experiment. The arrow indicates a cell selected for electroporation based on its modulation during spontaneous movements. Scale bar, 50 µm. b, Day 1, target cell (as indicated in a) before and after 2-PT guided electroporation with Alexa 594 and DNA (TVA, G, and mCherry). Note the entry of Alexa 594 into the target cell (red). Following electroporation, RV-RFP were injected close to the electroporated cell. Day 3, the target cell did not express the reporter protein mCherry, indicative of failed transfection. Note that we used a single plasmid encoding for TVA, G, and mCherry. Scale bar, 25 µm. c, Histological analysis of the example experiment (a-b). We did not observe the emergence of presynaptic cells (n = 4 brains). Red, RFP. Green, GCaMP6s. Blue, NeuroTrace 435/455. Scale bar, 1 mm.
