Extended Data Fig. 9: Spontaneous movements and neuronal properties of the movement-uncorrelated and movement-correlated groups for monosynaptic input tracing.
From: Brain-wide presynaptic networks of functionally distinct cortical neurons
a, Pie chart of time spent per spontaneous movement type (Wonly, WL) and not moving (nuncorr. and ncorr. = 11 mice; P > 0.05 for uncorr. vs. corr., 2-way ANOVA). b-g, Spontaneous movement parameters: Wonly frequency (b) and duration (c), WL frequency (d) and duration (e), mean (f) and maximum (g) locomotion speed (nuncorr. and ncorr. = 11 mice; P > 0.05 for a-g). In boxplots, the central line and box represent the median and 25th-75th percentiles, and the whiskers extend to the most extreme data points excluding outliers (larger than 1.5 × the interquartile range). h, Fraction of movement-uncorrelated, Movdown, and Movup neurons per group (nuncorr. and ncorr. = 11 mice; uncorr. vs. corr., P > 0.05, 2-way ANOVA). i, Cortical depth of each postsynaptic neuron. Depth was estimated starting from dura matter through in vivo 2-PT structural imaging (nuncorr. and ncorr. = 11 mice; P > 0.05, two-sided Wilcoxon rank-sum test). Inset, epifluorescence image of a coronal brain slice encompassing wS1 and a postsynaptic neuron expressing both a red and green fluorescent protein (arrow, n = 1 neuron, 1 mouse; postsynaptic neurons typically survive do not until the experimental endpoint). Red, RFP. Green, GCaMP6s/GFP. Cyan, DAPI. Scale bar, 100 µm.
