Extended Data Fig. 10: Long-range and local glutamatergic and GABAergic presynaptic neurons per brain.
From: Brain-wide presynaptic networks of functionally distinct cortical neurons
a, Number of long-range vs. total presynaptic neurons per brain (nuncorr. and ncorr. = 11 mice; P < 0.0001, regression). b, Long-range presynaptic neurons as fraction of total presynaptic neurons (nuncorr. and ncorr. = 11 mice; P > 0.05, two-sided randomization test). c, Epifluorescence image of a coronal brain slice, wS1 (example presynaptic network included in Fig. 3a,d; n = 22 mice). Note the “hourglass” distribution of RFP+ presynaptic neurons across cortical L2/3, L4, and L5. Scale bar, 250 µm. d, Images denoting the co-localization of RFP (presynaptic neurons, Alexa 555, red) and GABA (GABAergic cells, Alexa 647, represented in green) in wS1 (n = 22 mice). Scale bar, 50 µm. e-f, Glutamatergic (e) and GABAergic (f) presynaptic neurons as fraction of wS1 presynaptic neurons (nuncorr. = 10 mice; ncorr. = 11 mice; P > 0.05 for e-f, two-sided randomization tests). b, e-f, In boxplots, the central line and box represent the median and 25th-75th percentiles, and the whiskers extend to the most extreme data points excluding outliers (larger than 1.5 × the interquartile range).
