Extended Data Fig. 16: Behavioral effect of light presentation.
From: Brain-wide presynaptic networks of functionally distinct cortical neurons
a-c, Effect of light presentation on the spontaneous movements of control (n = 9, a), ArchT thalamus (n = 7, b), and ArchT M1/2 (n = 3, c) mice. Whisker movements aligned to the onset of light stimulation (mean ± s.e.m.). d, Whisker movements during baseline (n as in a-c; 0.5 s window prior to light onset; P = 0.048, Kruskal-Wallis test across mouse groups). e, Quantification of behavioral changes over the first part (left, 0–0.5 s) and second part (right, 0.5 – 1/1.5 s) of the light pulse relative to baseline. All groups showed a brief (first half) increase in whisker movements locked to light presentation onset (n as in a-c; control: P < 0.0072; ArchT thalamus: P < 0.0057; ArchT M1/2: P < 0.0020; one-sided Wilcoxon signed-rank test whisk. mov. norm ≠ 1 vs. whisk. mov. norm = 1), and this increase was comparable across groups (n as in a-c; P > 0.05, Kruskal-Wallis test). Whisker movements in the second half of the light pulse did not differ from that of baseline (P > 0.05 for each mouse group, one-sided Wilcoxon signed-rank test whisk. mov. ratio ≠ 1 vs. whisk. mov. ratio = 1) and were similar across groups (n as in a-c; P > 0.05, Kruskal-Wallis test). d-e, In boxplots, the central line and box represent the median and 25th-75th percentiles, and the whiskers extend to the most extreme data points excluding outliers (larger than 1.5 × the interquartile range).
