Fig. 3: Functionally distinct neurons have anatomically similar local presynaptic networks.
From: Brain-wide presynaptic networks of functionally distinct cortical neurons
a, Brain-wide monosynaptic input tracing. Top, schematics. Bottom, example two-photon images (n = 22 mice). Left to right: FOV (red arrows, postsynaptic PNs; white arrows, surrounding PNs; scale bar, 50 µm); postsynaptic PN immediately after electroporation (Alexa 594+; scale bar, 25 µm); postsynaptic PN 4 days after electroporation (mCherry+; scale bar, 25 µm); wS1 presynaptic neurons (RFP+; 3D projection of an 814 × 814 × 785 µm z-stack; scale bar, 100 µm). RV, rabies virus. b, Activity of the example postsynaptic (red) and surrounding (black) PNs aligned to WL onset (mean ± s.e.m.). c, Corresponding presynaptic network (individual symbols represent individual presynaptic neurons; colours represent anatomical regions). d, Mean modulation of postsynaptic neurons during WL. e, Number of presynaptic neurons per brain (P = 0.39, two-sided randomization test). f, wS1 versus total presynaptic neurons (P < 0.0001, regression). g, Fraction of presynaptic neurons in wS1 (P = 0.52, two-sided randomization test). h, Laminar distribution of wS1 glutamatergic presynaptic neurons (layers: L6 versus all other layers, P < 0.01, Kruskal–Wallis test (P < 0.0001) followed by Dunn’s tests with Bonferroni correction; uncorr. versus corr.: PL2/3 = 0.065, PL4 = 0.74, PL5 = 0.20, PL6 = 0.12, two-sided randomization tests). i, Laminar distribution of wS1 GABAergic presynaptic neurons (layers: L2/3 versus all other layers, P < 0.01, Kruskal–Wallis test (P < 0.0001) followed by Dunn’s tests with Bonferroni correction; uncorr. versus corr.: PL2/3 = 0.16; PL4 = 0.79; PL5 = 0.28, two-sided randomization tests). j, Horizontal, laminar projections of the weighted distributions of wS1 glutamatergic or GABAergic presynaptic neurons (uncorr. versus corr.: glutamatergic, PL2/3 = 0.73, PL4 = 0.39, PL5 = 0.066, PL6 = 0.26; GABAergic, PL2/3 = 0.86; PL4 = 0.84; PL5 = 0.31; two-sided randomization tests). Scale bar, 500 µm. d–g, Postsynaptic neurons (nuncorr. = 11; ncorr. = 11). In box plots, the central line and box represent the median and 25th–75th percentiles, and whiskers extend to the most extreme data points excluding outliers (larger than 1.5× the interquartile range). h–j, Postsynaptic neurons (nuncorr. = 10; ncorr. = 11).
