Extended Data Fig. 3: Suppression of cortical activity by TTX diffusion into the brain parenchyma using a custom cranial window.
From: Brain-wide presynaptic networks of functionally distinct cortical neurons
a-c, Example session. Top, Raster plots of neuronal activity before and at different time points after application of TTX. Neurons (184) are sorted from top to bottom by decreasing weight on the first principal component. Bottom, Corresponding whisker movements and locomotion speed traces. d, Modulation of individual neurons (1853) during spontaneous movements (Wonly + WL) before vs. after application of TTX (P > 0.05, regression). e, Prediction of whisker movements from population activity. Linear decoder predictive R2; the decoder was built using baseline data and evaluated on TTX out-of-sample data (n = 10 FOVs, 10 sessions, 10 mice, P > 0.05, one-sided paired sample t-test). f-k, Spontaneous movement parameters before and after application of TTX: Wonly frequency (f) and duration (g), WL frequency (h) and duration (i), mean (j) and maximum (k) locomotion speed (n = 10 sessions, 10 mice, P > 0.05 for all panels, two-sided Wilcoxon signed-rank test). f-k, In boxplots, the central line and box represent the median and 25th-75th percentiles, and the whiskers extend to the most extreme data points excluding outliers (larger than 1.5 × the interquartile range).
