Extended Data Fig. 5: Condensin facilitates the disassembly of structural loops during mitotic progression.
From: Extensive mutual influences of SMC complexes shape 3D genome folding
a, Schematic showing the RO-3306 arrest/release strategy to obtain condensin replete or deficient cells from G2 phase to mitosis. b, Flow chart showing the gating strategy to obtain pMPM2-negative, -low, -mid and -high populations from condensin-replete control samples. Two independent experiments were performed. c, Flow chart showing the gating strategy to obtain pMPM2-negative, -low, -mid and -high populations from condensin-deficient samples. Two independent experiments were performed. d, Representative images of DAPI signals from cells sorted in (b) and (c). Scale bar: 10 μm. Two independent experiments were performed. e, Upper panel: KR-balanced Hi-C contact maps showing the dynamic change of compartments when cells enter mitosis with or without condensin. Bin size: 100 kb. Lower panel: Saddle plots showing the preservation of compartments in condensin deficient cells during mitotic entry. f, KR-balanced Hi-C contact maps showing the dynamic change of structural loops (green arrow) in cells transiting from G2-phase to mitosis with or without condensin. Bin size: 10 kb. g, Bar graph showing the number of structural loops identified by HICCUPS in each population. h, APA plots showing the delayed disappearance of structural loops during mitotic entry without condensin. i, Bar graph showing the quantification of APA signals of structural loops during mitotic entry with or without condensin. Data are presented as mean values (n = 2 biological replicates for both conditions).
