Extended Data Fig. 7: Influence of cohesive- or/and extrusive-cohesin on mitotic chromosome architecture.
From: Extensive mutual influences of SMC complexes shape 3D genome folding
a, P(s) curve of the control mitotic cells. The three regimes were indicated by dotted lines. The secondary diagonal between regime (ii) and (iii) were indicated by arrow. b, Upper panel: P(s) curves of independent clones showing the influence of extrusive-cohesin (“heavy load”) on mitotic chromosome architecture. Lower panel: Zoom in P(s) curves showing the influence of heavily or lightly loaded extrusive-cohesin on mitotic chromosome architecture. c, P(s) curves of independent clones showing the influence of cohesive-cohesin on mitotic chromosome architecture. d, P(s) curves of independent clones showing the influence of cohesive-cohesin (G2/M short-arrest and release protocol) on mitotic chromosome architecture. e, KR-balanced Hi-C contact matrices for independent clones showing the log2 fold change of contact probabilities when cohesive-cohesin was loaded (G2/M short-arrest and release protocol). f, P(s) curves of independent clones showing the combined influence of cohesive-cohesin and extrusive-cohesin on mitotic chromosome architecture. g, P(s) curve showing gain of short-range contact frequencies in the condensin-deficient mitotic chromosomes by extrusive-cohesin. Note that light-loaded extrusive-cohesin displayed similar but milder effects. Curves for independent clone were shown. h, P(s) curve showing the influence of cohesive-cohesin alone on the condensin-deficient mitotic chromosomes. i, P(s) curves showing the effect of condensin II depletion on mitotic chromosome architecture. Data adapted from our previous study38. j, P(s) curves showing the effect of condensin I depletion on mitotic chromosome architecture. Data adapted from our previous study38.
