Extended Data Fig. 8: Extrusive- but not cohesive-cohesin influences the compartmentalization pattern of the condensin-deficient mitotic chromosomes.
From: Extensive mutual influences of SMC complexes shape 3D genome folding
a, KR-balanced Hi-C contact matrices of condensin-deficient mitotic chromosomes, showing the checkerboard compartmentalization patterns in condensin-deficient mitotic chromosomes with indicated configurations of cohesin. Bin size: 100 kb. b, Saddle plots of condensin-deficient mitotic chromosomes. c, KR-balanced Hi-C contact matrices showing loss of mA1 homotypic interactions (red arrows) in the presence of extrusive-cohesin (“heavy” or “light load”) but not cohesive-cohesin. Bin size: 24 kb. EV1 tracks were shown in parallel. d, KR-balanced Hi-C contact matrices showing the increased homotypic interactions among mB4 mitotic compartments in the presence of extrusive-cohesin (“heavy” or “light load”) but not cohesive-cohesin. Bin size: 100 kb. EV1 tracks were shown in parallel. mB4 compartments were marked by green bars. e, Aggregation-repulsion plots showing the impacts of extrusive-cohesin (“heavy-” or “light-load”) or/and cohesive-cohesin on mitotic chromosome compartmentalization patterns. Note that extrusive- but not cohesive-cohesin disrupted euchromatin (mA1, mA2) self-aggregation. f, Scatter plots showing the EV1 values of 25 kb genomic bins in the condensin-deficient mitotic chromosomes without any cohesin (x-axis) against condensin-deficient mitotic chromosomes with cohesive-cohesin, extrusive-cohesin (“heavy” or “light load”) or both (y-axes). Bins were color coded based on their compartment assignment. Green and red arrows indicate the reduction of EV1 values of bins within mB4 and mA1 compartments respectively.
