Extended Data Fig. 11: Structural loops are attenuated in G2 phase compared to late-G1 phase.
From: Extensive mutual influences of SMC complexes shape 3D genome folding
a, Flow cytometry plot showing the gating strategy to sort late-G1 and G2 phase cells from asynchronous population expressing the mCherry-MD cell cycle phase reporter. Two independent experiments were performed. b, Representative images showing the DAPI signal of sorted late-G1 or G2-phase cells. Scale Bar: 5 μm. Note that G2-phase cells displayed significantly larger nuclei size. P values were calculated using a two-sided Wilcoxon signed-rank test. n > 200 nuclei from two independent experiments. c, KR-balanced Hi-C contact maps showing the weakening of structural loops (indicated by green arrows) in G2-phase cells compared to late-G1. Bin size: 25 kb. Note that small structural loops were not affected (blue arrow). d, APA plots showing the dramatic attenuation of large structural loops in G2-phase compared to G1. e, Bar plot showing the APA signals corresponding to (d). Data are presented as mean values (n = 2 biological replicates for both conditions).
