Extended Data Fig. 9: The effects of ROS scavengers on the EasCCf reaction.
From: Chanoclavine synthase operates by an NADPH-independent superoxide mechanism
a, b, and d, The inhibitory data of ROS scavenger bilirubin, hydroxyl radical (HO•) scavenger mannitol and superoxide scavenger thiourea towards EasC activity. The enzyme activity of each sample is calculated as a percentage of the control sample. The individual (n = 3) and average values along with error bars (s.d.) of each group in one representative experiment among three independent experiments are shown. c, Restoration of the activity of tempol-inhibited EasC by buffer exchange. The reaction system is generally consisted of 20 μM EasC enzyme, 0.5 mM PCC, 2 mM NADPH, with different concentrations of ROS scavengers, respectively. The enzyme activity was measured by the remaining amount of the substrate.
