Extended Data Fig. 10: The competitive incorporation experiment using 18O-superoxide.
From: Chanoclavine synthase operates by an NADPH-independent superoxide mechanism
a, The pathway for SOTS-1 decomposition and its ensuing reaction with oxygen, leading to the release of superoxide. b, The illustration of the competitive incorporation experiment using 18O-superoxide in EasCCf reaction. c, The protocol for the artificial production of 18O-superoxide. 18O-superoxide was generated in the glove box by mixing the 18O2 with SOTS-1 in the reaction buffer. The buffer with 18O-superoxide was further moved to the open air to get rid of the 18O2. The control group follows the same protocol, except that SOTS-1 is not added. EasC noted in the figure is EasCCf.
