Extended Data Fig. 9: Immune territory of MYC amplified cells.
From: MYC ecDNA promotes intratumour heterogeneity and plasticity in PDAC
a, Bubble plot of the scaled average expression of LGR5 in epithelial cells of VR06. The colour intensity represents the expression level, and the size of the bubbles represents the number of expressing cells (left). Quantification of LGR5 expression in epithelial cells is provided on the right as mean ± SD of ROIs 3 and 4 (n = 3813) and ROIs 1 and 2 (n = 5729) of VR06. P values determined by Wilcoxon test (two-sided). b, Quantification of the WNT canonical ligand expression in CAFs as mean ± SD in ROIs 3 and 4 (n = 4919) and ROIs 1 and 2 (n = 2398) of VR06. P values determined by Wilcoxon test (two-sided). c, Bubble plot of the scaled average expression of LGR5 in epithelial cells of VR01. The colour intensity represents the expression level, and the size of the bubbles represents the number of expressing cells (left). Quantification of LGR5 expression in epithelial cells is provided on the right as mean ± SD of ROI 1 (n = 2031) and ROI 2 (n = 1398) of VR01. P values determined by Wilcoxon test (two-sided) (right). d, Quantification of WNT canonical ligands expression in CAFs as mean ± SD of ROI 1(n = 3318) and ROI 2 (n = 2811) of VR01. P values determined by Wilcoxon test (two-sided). e, Frequency of the annotated cell types across selected ROIs for each PDAC tissue. Areas containing MYC amplified cells (ecDNA+) are indicated. f, Proportion of cytotoxic T cells (CD8 expressing cells) per case stratified by presence or absence of MYC amplification. Statistical significance by Chi square (two-sided).
