Extended Data Fig. 1: Alveolar cells and fibroblasts exhibit differential signaling and enrichment during fibrosis and post-fibrotic resolution.
From: Histological signatures map anti-fibrotic factors in mouse and human lungs
(A) Specific ligand-receptor interactions between fibroblasts and either alveolar type II (ATII) or type I (ATI) cells. Fibroblast-ATII interactions at PID 14 are inferred to be largely similar to fibroblast-ATI interactions at PID 35 (red box). (B) Timepoint-specific differences in alveolar cells primarily consist of an established ATII-ATI transitional signature (Clu/Krt8/Krt18) enriched in ATI cells at PID 35. (C) Pdgfra, Col1a1, and Col3a1 expression by fibroblast subtypes. ECM-secreting fibroblasts (red boxes) exhibit notably higher average expression of Col1a1 and Col3a1 compared to other fibroblast subtypes. (D-F) Timepoint-dependent enrichment (D), top 3 differentially expressed genes (E), and top 10 gene ontology terms (F) of fibroblast subtypes. Red boxes indicate the relevant fibroblast subtype. P-values calculated based on the two-tailed Fisher’s exact test in enrichR.
