Extended Data Fig. 6: Current-voltage (I-V) plots from whole-cell recording and Linear regression relationships between Ca2+ induced fluorescent intensity (ΔFluorescence) and total charge transfer (Qtotal) of whole-cell current recording.
From: GluA2-containing AMPA receptors form a continuum of Ca2+-permeable channels
a-d show the I-V relationships recorded using a RAMP protocol, from −100mV to +60 mV, with whole-cell configuration for GluA1/A2 (black, n = 11), GluA1/A2γ2 (orange, n = 12), GluA1/A2γ2/CNIH-3 (cyan, n = 15) and GluA1/A2R607Eγ2 (dark blue, n = 12), respectively. The shades of each I-V plot indicates the SEM. Noted that GluA1/A2R607Eγ2 receptors are sensitive to internal polyamines with an affinity of Kd(0mV) value of 18.7 ± 3.0 μM. e-h show the linear regression relationships between Ca2+ induced fluorescent intensity (ΔFluorescence) and total charge transfer (Qtotal) of whole-cell current recording for GluA1/A2 (grey, n = 11), GluA1/A2γ2 (orange, n = 12), GluA1/A2γ2/CNIH-3 (cyan, n = 15) and GluA1/A2R607Eγ2 (dark blue, n = 12), respectively. Scatters indicate individual data points, while the solid lines are the linear regression for each group: GluA1/A2: y = 0.0173*x + 65.935, r = 0.56; GluA1/A2γ2: y = 4.8557*x-4068.4, r = 0.94; GluA1/A2γ2/CNIH-3: y = 3.4786*x-566.31, r = 0.87 and GluA1/A2R607Eγ2: y = 7.2528*x, r = 0.88.
