Extended Data Fig. 2: TARPs promote Ca2+ permeability of GluA2-containing AMPARs while remaining polyamine insensitive.
From: GluA2-containing AMPA receptors form a continuum of Ca2+-permeable channels
a. Different AMPAR-TARP complexes arranged according to the reversal potential observed in 108 mM external Ca2+ solution (A1/A2: ErevCa2+ = −44.8 ± 2.8 mV, n = 6; A1γ8/A2: ErevCa2+ = −29.7 ± 1.8 mV, n = 5; A1/A2γ8: ErevCa2+ = −25.6 ± 2.9 mV, n = 5; A2γ2/A3γ2: ErevCa2+ = −31.4 ± 2.3 mV, n = 11; A1γ2/A2: ErevCa2+ = −31.5 ± 1.2 mV, n = 5; A1/A2γ2: ErevCa2+ = −7.2 ± 1.8 mV, n = 16; A1γ2/A2γ2: ErevCa2+ = −27.0 ± 1.8 mV, n = 6). b. Pooled data of PCa/PNa of AMPAR-TARP complexes co-assembled with TARP γ2 or γ 8 (A1/A2 PCa/PNa = 0.06 ± 0.01, n = 6; A1γ8/A2 PCa/PNa = 0.12 ± 0.01, n = 5; A1/A2γ8 PCa/PNa = 0.18 ± 0.03, n = 5; A2γ2/A3γ2 PCa/PNa = 0.12 ± 0.01, n = 11; A1γ2/A2 PCa/PNa = 0.12 ± 0.01, n = 5; A1/A2γ2 PCa/PNa = 0.43 ± 0.04, n = 16; A1γ2/A2γ2 PCa/PNa = 0.14 ± 0.01, n = 6). Two-sided Kruskal-Wallis ANOVA followed by Mann-Whitney U tests with Bonferroni-Holmes correction. p-value * <0.05, ** <0.01, *** <0.001. c. IV plots of different AMPAR-TARP complexes in external 150 mM Na+ (open circles fitted by a 4th order polynomial function) solution. d. GV plots of AMPAR-TARP complexes obtained from IV curves above (c). Open circles show average normalized response and colored shading show s.e.m. (A1/A2: n = 6; A1γ2/A2: n = 5; A1/A2γ2: n = 16; A1γ2/A2γ2: n = 6).
