Extended Data Fig. 3: Simulations of current-voltage (IV) and conductance-voltage (GV) plots establishes that data from edited GluA2-containing AMPARs is not impacted by unedited AMPARs.

a. GV plots of unedited GluA2(Q) AMPARs showing that tethering TARP γ2 significantly attenuates the degree of block by cytoplasmic spermine (30 μM). Adapted from the data shown in Supplementary Table 3. b. A series of GV plots where the contribution (%) of unedited AMPARs to the overall AMPAR response is reduced in a stepwise manner. Note that shape of the GV plot with only 1 % contribution of unedited AMPARs best fits the data we have observed with GluA1/A2 heteromers. c. A series of Ca²⁺ IV plots where the contribution (%) of unedited AMPARs to the overall AMPAR response was adjusted in an incremental manner. Note, that at least 50% of unedited AMPARs would be needed for the reversal potential to be in the positive voltage range. d. The relative Ca²⁺ permeability of AMPARs estimated from the simulated Ca²⁺ IV plot data shown in c. Note, that for the relative Ca²⁺ permeability to be close to 1 (dotted line), as described for GluA1/A2 heteromers with TARP γ2 and CNIH3, almost 75 % of the overall response would need to contain unedited AMPARs.

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