Extended Data Fig. 9: Time-Course Analysis of BRCA2, RAD51 and PARP1 recruitment in HR repair.
From: BRCA2 prevents PARPi-mediated PARP1 retention to protect RAD51 filaments
(a) Representative epifluorescence (inset) and super-resolution SMLM-STORM images of a single nucleus, post damage, and stained for naDNA (yellow), RAD51 (cyan) and BRCA2 (magenta). Scale bar = 2 μm. (b) Spatial and temporal mapping of BRCA2, RAD51 and PARP1 proteins respectively, over 16 h of recovery post damage. Values on the graph represents the percent change in localization of BRCA2, RAD51 and PARP1 to naDNA compared to (nontreated) NT cells. Data shown is mean ± s.d (r = 2). (c-e) Kinetics of BRCA2, RAD51 and PARP1 localizing at nascent DNA throughout repair (over 16 hr of recovery for BRCA2-RAD51 and 5 h recovery for PARP1). For all quantification graphs individual data points represent result from single cell. Values on graph indicate P-values of unpaired two-sample t-tests between NT, CPT-treated and CPT followed by PARPi treated cells. Cartoons were created in BioRender. Lahiri, S. (2025) https://biorender.com/a58b868. *P < 0.05, **P < 0.01, and ***P < 0.001 and ****P < 0.0001. [CPT] = 0.1 µM.
