Extended Data Fig. 1: General Quality control of SPIDR screen.
From: Comprehensive interrogation of synthetic lethality in the DNA damage response
Quantification of log2 pair count of replicate 1 at a, T0 and b, T14 shows high coverage at T0 and drop out of many combinations at T14. c, LFC comparison between library elements (targeting:non-targeting) with the targeting sgRNA in library position A or B in screen replicate 1 and 2. d, log(RPM + 1) analysis of screen replicate count data at T0 and T14. e, LFC comparison T14vsT0 for all library elements. f, Volcano plot of log2 fold change of non-targeting (non-targeting:non-targeting) and single gene (targeting:targeting) targeting library elements at T14 compared to T0. Common essential genes (e.g., MCM2, GTF2H2) show high negative values while tumor suppressors SAMHD1 and TAOK1 show a positive log2 fold change, respectively. Statistical significance was determined using the Wald test, with Benjamini-Hochberg post-test.
