Extended Data Fig. 3: Orthogonal validation of novel synthetic lethal gene pairs.
From: Comprehensive interrogation of synthetic lethality in the DNA damage response
a, Left: Schematic representation of the dual-color flow cytometry assay. Cells are transduced with sgRNAs and assayed 96 h later and at regular intervals thereafter. Right: An example of the flow cytometry gating strategy. b, Representative flow cytometry density plots of the FEN1:WDR48 interaction in RPE-1 TP53 KO dCas9-KRAB cells. c-h, RPE-1 TP53 KO dCas9-KRAB cells were co-transduced with the indicated sgRNAs. Cells populations were first measured by flow cytometry 96 h after transduction (day 0) and on the indicated days thereafter. Log2 fold changes relative to day 0 are shown. These panels show the full quantifications of data plotted in Fig. 1e.
