Fig. 2: Water and Mg2+ ion detection with SWIM and consensus between 2.2 Å and 2.3 Å maps.
From: Complex water networks visualized by cryogenic electron microscopy of RNA
a,b, Detected waters (red) and Mg2+ ions (green) for the 2.2 Å (a) and 2.3 Å (b) model. Consensus water and Mg2+ ions coloured dark and counts are noted next to each model. c–e, Distributions of water and Mg2+ ions separated by consensus and non-consensus types: Q score of water or Mg2+ ions (c; PMg = 5.7 × 10−2 and Pwater = 6.1 × 10−4), average Q score of bound RNA nucleotides (d; PMg = 2.2 × 10−3 and Pwater = 1.0 × 10−6) and RMSD of bound RNA nucleotides between the 2.2 Å and 2.3 Å models after alignment on all RNA heavy atoms (e; PMg = 2.6 × 10−2 and Pwater = 3.8 × 10−5). The horizontal line is the mean value. Pairwise significance was determined by a two-sided Mann–Whitney U-test: not significant (NS) P > 0.05, *P < 0.05 and **P < 10−4.
