Extended Data Fig. 5: HHO xenotransplantation.
From: Generation of human adult hepatocyte organoids with metabolic functions
a. Concentration of human albumin in mouse sera following xenotransplantation of PM11 HHOs. HHOs with different passage counts are shown in different colors. n shows the number of mice analyzed. b. STEM121 staining of PM11 HHO xenografts. Scale bar: 1 mm. Quantification shown in Extended Data Fig. 5c. c. Repopulation efficiency of PM11 PHHs and eHHO with different culture duration. Each dot denotes one mouse. Statistics, one-way ANOVA followed by Dunnett’s test with the control (PHHs). d. Concentration of human albumin in mouse sera following xenotransplantation of PM16 HHOs. HHOs with different passage counts are shown in different colors. e. Repopulation efficiency of PM16 PHHs and eHHO with different culture duration. Each dot denotes one mouse. Statistics, one-way ANOVA followed by Dunnett’s test with the control (PHHs). f. Repopulation efficiency of PM10, PY9, PY12 eHHO, dHHO and dHHO+WR. Each dot denotes one mouse. g. Concentration of human albumin in mouse sera following xenotransplantation of PY39a HHOs. h. STEM121 staining of PY39a HHO xenografts. Scale bar: 1 mm. Quantification shown in Extended Data Fig. 5i. i. Repopulation efficiency of PY39a PHHs and eHHO with different culture duration. Each dot denotes one mouse. Statistics, one-way ANOVA followed by Dunnett’s test with the control (PHHs). j. The length of telomere in HHOs and their parental PHHs after prolonged culture. Telomere length was measured with quantitative real-time PCR. Data from PHHs and HHOs derived from four different donors. Data are shown as mean ± SD. k. Representative Ki67 (red) staining in a STEM121-positive (light blue) HHO xenograft in the mouse liver. Nuclear counterstaining, Hoechst 33342 (green). Scale bar: 20 μm. Quantification shown in Extended Data Fig. 5l. l. The relationship between the repopulation efficiency and percentage of Ki67+ cells in xenograft. Each dot shows one mouse. One section slide was analyzed per mouse. r, Pearson correlation coefficient, p = 0.8801, t-statistics, two-sided. m. Area overlap ratio between hMRP2 and hALB. (related to Fig. 2i). For each PHH or HHO, two to three mice were analyzed, and the ratio between hALB+ area and hMRP2+ area was calculated. Each dot shows the mean ratio. One section slide was analyzed per mouse. n. Representative images of a xenograft in the PV-CV area stained for the PV zone marker ASS1 and STEM121. Nuclear counterstaining, Hoechst 33342. Scale bar: 100 μm. Hatched areas indicate the venous lumens. *Indicates PV lumen. Representative data from three donors with similar results. o. Representative immunostaining images of CV area with the CV zone marker GS (red), CD31 (blue) and STEM121 (green). Scale bar: 100 μm. Similar results were obtained from three donors.
