Extended Data Fig. 5: Single cell characterization of NRXN1+/− organoids.
From: Phenotypic complexities of rare heterozygous neurexin-1 deletions
(a, f), UMAPs of hCO and hSO organoid samples sequenced at 6 months, annotated by cell clusters. (b, g) Extended gene expression panel across sub-clusters of hCO and hSO samples across neuronal, cortical, subpallial and astroglia markers. (c, h) Validation of regionalization across forebrain (FOXG1), dorsal (EMX1) and ventral (DLX2) regions, with NRXN1 expression across all cells. (d, i) Relative proportions of cell clusters across pooled and individual genotypes, (hCO = 47,460 cells) and (hSO = 35,563 cells). (e, j) Gene ontological analysis results using DEGs from scRNASeq. *P < 0.05, **P < 0.01, ***P <0.001, two-tailed Wilcoxon’s rank sum test, FDR = 0.05.
