Fig. 3: Characterization of the gp mutant.

a, General view of near-isogenic plants (BC6 S1 generation from the cross JI0015 gp/gp x Caméor Gp/Gp) developed in this study. Pot diameter is 9 cm. b, Transmission electron microscopic (TEM) sections of pod mesocarp cells. Scale bars, 1 μm. c, TEM sections of leaflet spongy mesophyll cells. Note the poorly developed thylakoid membranes (arrows) in gp compared to Gp. Scale bars, 0.5 μm. d, An approximately 100-kb genomic deletion adjacent to ChlG is illustrated for gp compared to the reference genome ZW6 (Gp). The deletion event in gp lines is illustrated on the Gp reference genome by the dashed box. The approximately 100-kb deletion event was called according to genome assembly comparisons between JI0015 and JI2822 (ref. ⁶²). Far right, expression of transcript types T0 and T1–T5 (Gp/Gp wild type; top right) and t0 and t1–t6 (gp/gp mutant; bottom right); numbers at ends of (or in) bars indicate the expression (in transcripts per million (TPM)). More details are provided in Supplementary Figs. 15–19 and Supplementary Tables 23–27. e, Crossing scheme for a complementation test between Caméor M4 TILLING line 411.1 carrying one lethal allele of ChlG and gp (JI0015), with the two types of expected F₁ genotype. ChlG^WT and ChlG^W121* represent the wild-type and TILLING alleles of ChlG. WT represents the presence of the wild-type (Caméor) sequence between ChlG and the TIR-NBS-LRR gene, and Δ^gp represents the approximately 100-kb deletion, which co-segregates with gp. The question being addressed is whether ChlG^W121*-WT complements gp (ChlG^WT-Δ^gp). f, F₁ pods segregating for green versus yellow. The number after the underscore is the plant number; the parental lines (TILL_6 het and JI0015) and wild-type Caméor are also shown. g, Codominant PCR marker test confirming that all plants presumed to be F₁ are Gp/gp heterozygotes (top) and a dCAPS marker PCR test confirming that only the yellow-podded F₁ plants inherited the ChlG^W121* TILLING allele (bottom). M, DNA size marker (0.5 – 3 kb; 100 bp ladder lane from 1 kb to 0.5 kb and below).