Extended Data Fig. 4: Anatomical and molecular characterization of pod endocarp development and associated pathways.
a-c, Microscopic imaging showing the anatomical patterns of the pod endocarp in wildtype (P/P V/V, JI2776) at different developmental stages: 3 days, 8 days, and 12 days post flowering. d, Gene expression levels, measured by RNA-seq approach, of key genes involved in the conserved well-established TDIF-PXY-WOX signalling pathway (PsCLE41, PsSERK, PsPXY, PsWOX4, PsWOX14) and two key component genes (PsMYB26, PsNAC) which have been reported to be involved in the secondary cell wall thickening and lignification in Arabidopsis. Expression data were obtained from various organs at different developmental stages (three biological replicates for each sample) in Caméor. The normalization and statistical approach was the same as described in Extended Data Fig. 2h. e, Comparative microscopic imaging of pod endocarp anatomical patterns at 12 days post flowering in four genotypes: P/P V/V (JI0190), p/p V/V (JI0466), P/P v/v (JI0074), and p/p v/v (JI0134). f, qRT_PCR analysis of PsCLE41, PsPXY, PsMYB26, and PsNAC in lines with different genotypes (P/P V/V, p/p V/V, P/P v/v, p/p v/v). The pod samples were obtained 8 days post flowering. Two different lines were selected for each genotype, with five biological replicates (n = 5) for each sample. Data are presented as mean ± SEM, and statistical significance was determined using a two-sided t-test (which applies to g). g, qRT_PCR results of PsMYB26 and PsNAC compared between the control and the VIGS-silenced lines. Three to five biological replicates were used for each sample both in the control and silenced lines. h, Proposed model illustrating the functional roles of PsCLE41 and PsMYB26 in pod endocarp development and lignin biosynthesis.
