Extended Data Fig. 2: Interaction of BMAL1 and HIF2A.
From: BMAL1–HIF2A heterodimer modulates circadian variations of myocardial injury
a, GO enrichment analysis was conducted to elucidate the molecular functions (MF), biological processes (BP), and cellular components (CC) associated with proteins predicted by the HuRI to potentially interact with BMAL1; two-sided Fisher’s exact test, Benjamini–Hochberg correction applied. b, Western Blot analysis of reciprocal co-IP with HIF2A in hypoxia-treated (1% O2, 4 h) or normoxia-treated HEK293 cells. Cytosolic and nuclear protein extracts were immunoprecipitated with HIF2A and blotted with anti-BMAL1, anti-HIF2A, anti-HIF1B, anti-β-actin, and anti-Lamin A/C antibodies. An IgG control affirmed procedure specificity. H indicates HIF2A. n = 3 independent experiments. c, Size-exclusion chromatography analysis of the BMAL1/HIF2A heterodimer. The purified BMAL1/HIF2A complex was loaded onto a Superdex 200 Increase 10/300 GL column. n = 3 independent experiments. The molecular weights of makers are as indicated. d, SDS-PAGE analysis of the recombinant BMAL1/HIF2A heterodimer purified by size-exclusion chromatography. n = 3 independent experiments.
