Extended Data Fig. 5: A memory of a cold-paired context is sufficient to increase metabolic rate and alter behavior in females.

a Time plot of oxygen consumption between female mice during Baseline 1 (21 °C; yellow), Test 1 (21 °C; purple) and Cold 1 (4 °C; dark blue; left), with comparisons at hour 2, hour 4, hour 6 and total time averaged in metabolic cages (right). b Time plot of carbon dioxide production between female mice during Baseline 1 (21 °C; yellow), Test 1 (21 °C; purple) and Cold 1 (4 °C; dark blue; left), with comparisons at hour 2, hour 4, hour 6 and total time averaged in metabolic cages (right). c Time plot of energy expenditure between female mice during Baseline 1 (21 °C; yellow), Test 1 (21 °C; purple) and Cold 1 (4 °C; dark blue; left), with comparisons at hour 2, hour 4, hour 6 and total time averaged in metabolic cages (right). d Time plot of movement between female mice during Baseline 1 (21 °C; yellow), Test 1 (21 °C; purple) and Cold 1 (4 °C; dark blue; left), with comparisons at hour 2, hour 4 and total time averaged in metabolic cages (right). e Time plot of food consumption between female mice during Baseline 1 (21 °C; yellow), Test 1 (21 °C; light blue) and Cold 1 (4 °C; dark blue; left), with comparisons at hour 2, hour 4 and total time averaged in metabolic cages (right). a-e Data shown as mean ± SEM, n = 7-8 mice per group. a-e, Repeated measures ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. BL1, baseline 1; T1, Test 1; CL1, cold 1; VO₂, oxygen consumption; VCO₂, carbon dioxide emission; EE; energy expenditure; h, hour.