Extended Data Fig. 7: Innate cold exposure, but not stress, increases lipolytic and thermogenic gene expression in BAT.
From: Cold memories control whole-body thermoregulatory responses
a Diagrammatic representation of the thermogenic pathway in BAT (above), with experimental timeline for tissue collection (below). Relative expression of lipolysis and thermogenesis genes b, Ucp1, c, Cpt1α, d, Cact, e, Atgl, f, Hsl and g, Pgc1α from the BAT of mice housed during BL1 (grey) compared cold exposed mice (CL1; blue). h, Diagrammatic representation of experimental timeline for tissue collection. Relative expression of lipolysis and thermogenesis genes i, Ucp1, j, Cact, k, Cpt1α, l, Atgl, m, Hsl and n, Il6 from the BAT of mice that received foot shocks compared to no shock controls. n = 6-7 mice per group. o Diagrammatic representation of experimental timeline for tissue collection. Relative expression of lipolysis and thermogenesis genes p, Ucp1, q, Cact, r, Cpt1α, s, Atgl, t, Hsl and u, Il6 from the BAT of mice that were exposed to TMT and H2O exposed controls. b-g,i-n,p-u, Data shown as mean ± SEM n = 3-7 mice per group. b-g,i-n,p-u, unpaired t-test. *p < 0.05, **p < 0.01. BAT, brown adipose tissue; FFA, free fatty acid; BL1, baseline 1; CL1, cold 1; m, minutes; NS, no shock; S, shocked; TMT, trimethylthiazoline; h, hour. Schematics in a, h and o created with BioRender (https://biorender.com).
