Extended Data Fig. 9: Distinct stress-related stimuli activate divergent brain regions.

a Diagrammatic representation of experimental timeline for tissue collection. b Representative image of hippocampal slice with Fos⁺ cells (red) and DAPI⁺ cells (blue). Comparison of Fos⁺ neurons normalized to area in the c, DG d, CA3 e, CA1 f, LHA g, DMH h, VMH i, ARH j, PVi k, SSp l, BLA m, BMA and n, CeA of mice that received foot shocks (red) compared to no shock controls (grey). Data shown as mean ± SEM, c-n, n = 5-6 mice per group. c-n, unpaired t-test. *p < 0.05, **p < 0.01, ***p < 0.001. S, shocked; NS, no shock; DG, dentate gyrus; CA3, cornu ammonis 3; CA1, cornu ammonis 1; LHA, lateral hypothalamic area; DMH, dorsomedial nucleus of the hypothalamus; VMH, ventromedial hypothalamic nucleus; ARH, arcuate hypothalamic nucleus; PVi, periventricular hypothalamic nucleus-intermediate part; SSp, primary somatosensory area; BLA, basolateral amygdalar nucleus; BMA, basomedial amygdalar nucleus; CeA; central amygdalar nucleus. Schematic in a created with BioRender (https://biorender.com).