Fig. 2: Contextual cold memories are encoded and retrieved in the hippocampus and hypothalamus.

a, Experimental timeline for tissue collection. b, Automated brain-wide FOS detection pipeline used to identify FOS⁺ cells in multiple brain regions simultaneously. iDISCO, immunolabelling-enabled imaging of solvent-cleared organs. c, Hippocampal slice (left) with FOS⁺ cells (red; bottom right) and DAPI⁺ cells (blue; top right). Scale bar, 500 µm. d–f, Comparison of FOS⁺ neurons normalized to area in the DG (d), CA3 (e) and CA1 (f) between mice during BL1 (white), CL1 (dark blue) and T1 (light blue). g, Correlation between FOS⁺ cells in the DG and oxygen consumption between mice during BL1 (white), CL1 (dark blue) and T1 (light blue). h–j, Comparison of FOS⁺ neurons normalized to area in the LHA (h), MPO (i) and LPO (j) between mice during BL1 (white), CL1 (dark blue) and T1 (light blue). k, Correlation between FOS⁺ cells in the LHA and oxygen consumption between mice during BL1 (white), CL1 (dark blue) and T1 (light blue). l–n, Correlation analysis of FOS⁺ cells between brain regions at BL1 (l), CL1 (m) and T1 (n). Correlations with R > 0.5 are displayed in red. Correlations with R < 0.5 are displayed in grey. Line thickness is proportional to the strength of the correlation (R value). d–f,h–j, Data are mean ± s.e.m., d–n, n = 7–10 mice per group. d–f,h–j, One-way ANOVA. g,k, Simple linear regression with slope comparison. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Illustration of the tissue collection a and schematic in b created with BioRender (https://biorender.com).