Fig. 5: Inhibition of cold-sensitive engrams prevents the memory effects on oxygen consumption.
From: Cold memories control whole-body thermoregulatory responses
a, Genetic strategy. b, Representative image of a hippocampal slice with FOS+ cells (red) and mCitrine+ cells (green). Scale bar, 250 μm. c, Experimental timeline. d, Time plot of oxygen consumption of saline-injected mice during BL1 (grey) and T1 (teal). e–g, Comparison of oxygen consumption of saline-injected mice during BL1 (grey) and T1 (teal) at hour one, hour two and total time averaged. h, Time plot of oxygen consumption of CNO-injected mice during BL1 (grey) and T1 (teal). i–k, Comparison of oxygen consumption of CNO-injected mice during BL1 (grey) and T1 (teal) at hour one, hour two and total time averaged. l, Time plot of the percentage change in oxygen consumption on T1 (teal) compared with BL1 (black) in saline-injected mice. m, Time plot of the percentage change in oxygen consumption on T1 (teal) compared with BL1 (black) in CNO-injected mice. n, Comparison of the percentage change in oxygen consumption between saline-injected (grey) and CNO-injected (teal) mice on T1. Data are mean ± s.e.m., d–n, n = 7–8 mice per group. e–g,i–k, Paired t-test. n, Unpaired t-test. **P < 0.01, ***P < 0.001. Illustration of the transgenic labelling system (a) and the behavioural timeline (c) were created with BioRender (https://biorender.com).
