Extended Data Fig. 2: LSD1 and GSK3 inhibitors potently synergize in different AML cell lines.
From: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
a. Synergy plot using an HSA model for THP-1, Kasumi-1, U937, MOLM-13, OCI-AML3 and OCI-AML2 cells treated with different concentrations of GSK-LSD1 and LY2090314 for 5 days. b, Analysis of CD11b mRNA relative levels in U937 cells treated with the indicated inhibitors for 5 days. Values were normalized against GAPDH. Data are presented as mean ± SD of 3 biological independent experiments (n = 3). P-values were determined using two-way analysis of variance (ANOVA). c, Analysis of CD11b cell-surface protein relative levels in U937 cells treated with the indicated inhibitors for 5 days. Data are presented as mean ± SD of 3 biological independent experiments (n = 3). P-values were determined using two-way analysis of variance (ANOVA). d, Analysis of CD11b mRNA relative levels in human AML cells treated with the indicated inhibitors for 5 days. Values were normalized against GAPDH. Data are presented as mean ± SD of 3 biological independent experiments (n = 3). P-values were determined using two-way analysis of variance (ANOVA). e, Analysis of THP-1 cell viability after treatment with IMG-7289, LY2090314 or combination of both inhibitors for 5 days by Cell-Titer-Glo assay. Data are presented as mean ± SD of 3 biological independent experiments (n = 3). P-values were determined using two-way analysis of variance (ANOVA). f, Analysis of CD11b mRNA relative levels in THP-1 cells treated with IMG-7289, LY2090314 or combination of both inhibitors for 5 days. Values were normalized against GAPDH. Data are presented as mean ± SD of 3 biological independent experiments (n = 3). P-values were determined using two-way analysis of variance (ANOVA). g, Analysis of THP-1 cell viability after treatment with TAK-418, LY2090314 or combination of both inhibitors for 5 days by Cell-Titer-Glo assay. Data are presented as mean ± SD of 3 biological independent experiments (n = 3). P-values were determined using two-way analysis of variance (ANOVA). h, Analysis of CD11b mRNA relative levels in THP-1 cells treated with TAK-418 or LY2090314 and combination of both inhibitors for 5 days. Values were normalized against GAPDH. Data are presented as mean ± SD of 3 biological independent experiments (n = 3). P-values were determined using two-way analysis of variance (ANOVA).
