Extended Data Fig. 4: Combo treatment activates type I interferon signaling in AML cells.
From: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
a, b, c, Analysis of ISG15 and MX1 mRNA relative levels in THP-1 (a), MOLM-13 (b) and U937 (c) cells treated with indicated inhibitors. Values were normalized against GAPDH. Data are presented as mean ± SD of 3 biological independent experiments (n = 3). P-values were determined using two-way analysis of variance (ANOVA). d, CUT&RUN-qPCR occupancy analysis of β-catenin on promoter of STAT1, IFIH1 and STAT2 in OCI-AML3 cell treated with indicated inhibitors. Data are presented as mean ± SD of 3 biological independent experiments (n = 3). P-values were determined using two-way analysis of variance (ANOVA). e, CUT&RUN-qPCR occupancy analysis of IRF7 on promoter of STAT1, IFIH1 and STAT2 in OCI-AML3 cell treated with indicated inhibitors. Data are presented as mean ± SD of 3 biological independent experiments (n = 3). P-values were determined using two-way analysis of variance (ANOVA). f, CUT&RUN-qPCR occupancy analysis of β-catenin on promoter of STAT1, IFIH1 and STAT2 in MOLM-13 cell treated with indicated inhibitors. Data are presented as mean ± SD of 3 biological independent experiments (n = 3). P-values were determined using two-way analysis of variance (ANOVA). g, CUT&RUN-qPCR occupancy analysis of IRF7 on promoter of STAT1, IFIH1 and STAT2 in MOLM-13 cell treated with indicated inhibitors. Data are presented as mean ± SD of 3 biological independent experiments (n = 3). P-values were determined using two-way analysis of variance (ANOVA). h, Scatter correlation plot of H3K4me1 and mRNA expression in THP-1 cells treated with combo. R indicates Pearson correlation value. “ns” indicates not significant.
