Extended Data Fig. 5: Functional analysis of motifs within post-transcriptional repressors.
From: Deciphering disordered regions controlling mRNA decay in high-throughput
a, Western blot of wildtype and motif-mutated, V5-tagged Tis11 from log-phase cells grown in complete synthetic media. Representative result from n = 2. b, Growth curves for wildtype, motif mutant, and ∆tis11 in complete synthetic media alone (no ferrozine) and c, with 750 µM ferrozine and 300 µM ammonium iron(II) sulfate. Different shades correspond to biological replicates (n = 3). d, Fragments from Eap1 in wildtype disordered library screen, coloured as in Fig. 3c. Individual measurements are shown as points (n = 2). The Y(X4)Lφ and repressive motif regions are highlighted in purple. e, Sequence alignment from representative yeast species of conserved sequences within the repressive region of Eap1. Motif-1 and Motif-2 are displayed and correspond to naming in Fig. 4g,h. E. cym, Eremothecium cymbalariae; V. pol, Vanderwaltozyma polyspora; T. pha, Tetrapisispora phaffii; K. lac, Kluyveromyces lactis.
