Extended Data Fig. 3: Depletion of core mRNA decay nucleases and activity scores in depleted backgrounds.
From: Deciphering disordered regions controlling mRNA decay in high-throughput
a-d, Growth curves of reporter strains expressing a, the Tir1 F-box protein only, or with the mAID tag on b, Ccr4 c, Pop2 or d, Dcp2. All strains were treated with 500 µM IAA or 1% DMSO as indicated. Different shades correspond to individual biological replicates (n = 3). e, Fitted growth rates for the curves shown in a-d. Mean of fits for individual biological replicates and standard deviation are displayed (n = 3). f-h, Correlation between activity scores for biological replicates of tethering library in depletion strains after ~16 h of IAA treatment for f, Ccr4 depletion g, Pop2 depletion and h, Dcp2 depletion, with Pearson correlation coefficients shown in panel legends. i-j, Comparison of average activity scores in the wildtype strain and average activity scores in strains where nucleases have been depleted. i, Ccr4 j, Dcp2 (n = 2). Repressors with significant difference in activity are shown in red. Grey lines demarcate statistical significance (see Methods, padj ≤ 0.05).
