Extended Data Fig. 4: Sort-seq of mutational scanning library and activity scores.
From: Deciphering disordered regions controlling mRNA decay in high-throughput
a, Distribution of YFP:RFP ratios of reporter yeast expressing the mutational scanning library. Vertical lines demarcate equal population bins used in sorting. The distribution is more uniform than the proteome-wide library because these tethering constructs are derived from highly repressive peptides. b,c, Correlation between b, stability scores and c, activity scores for biological replicates of the mutational scanning screen. The Pearson correlation coefficient is displayed in panel legend. d, Correlation between average activity scores for wildtype repressive fragments in the mutational scanning library screen and for these peptides in proteome-wide disordered library screen (n = 2). Repressors are shown in black and 20 inactive controls shown in red. e, Cumulative probability of the conservation scores of fragments or motifs. Conservation was calculated from multiple sequence alignments of each protein from the yeast genome order browser and per-residue conservation scores were determined (see Methods). Only residues within motifs are significantly different from the other distributions (Kolmogorov-Smirnov test p-value < 0.05).
