Extended Data Fig. 8: ALPK1 expression in human MDS/AML and Dnmt3a mutant HSPCs.
From: Microbial metabolite drives ageing-related clonal haematopoiesis via ALPK1
a, Enrichment of MDS- and AML-associated mutations in patients based on high or low levels of ALPK1 (Z score). b, Immunoblotting of BM CD34+ cells isolated from healthy donor (n = 1) and MDS patients (n = 2) stimulated with ADP-heptose (1 µg/ml) for 30 min. c, ALPK1 and TIFA mRNA expression in Dnmt3aWT and Dnmt3aKO HSC from the publicly available dataset GSE98191 (n = 3 mice per group). d, Relative mRNA expression of Tifa in purified HSCs isolated from matched Dnmt3aWT and Dnmt3aKO (n = 5 mice per group) or Dnmt3aWT and Dnmt3aR878H mice (n = 3 mice per group). e, Quantification of immunoblotting from Fig. 3p (n = 2 independent experiments). f, Immunoblot analysis of HSPCs from WT, Alpk1KO, Dnmt3aKO, and Dnmt3aKO;Alpk1KO mice stimulated with indicated concentrations of ADP-heptose for 30 min to examine MAPK signalling (n = 2 independent experiments). For gel source data, see Supplementary Fig. 1. Error bars represent the SEM. *, P < 0.05, **, P < 0.01, ***, P < 0.001. P values were calculated for the indicated comparisons using a two-tailed unpaired Student’s t-test (c, d).
