Extended Data Fig. 6
From: Dopamine D1–D2 signalling in hippocampus arbitrates approach and avoidance
Calcium imaging of vSub D1 and D2 excitatory neuron activity during EPM testing. a, Experimental schematic (n = 6 D1-Cre, n = 6 D2-Cre male mice). b, Representative images at the fiber tip (FT) in a D1-Cre (left) and D2-Cre (right) mouse vSub. GCaMP6f-positive cells in magenta if CaMKIIα+|GAD67−, in cyan if CaMKIIα−|GAD67+, in yellow if CaMKIIα+|GAD67+. EC entorhinal cortex. c, Quantification in D1-Cre (top, 1 hemisection/mouse) and D2-Cre (bottom, 1 hemisection/mouse) animals indicating GCaMP6f expression largely restricted to excitatory CaMKIIα+|GAD67− cells. d, Representative D1-Cre (top) and D2-Cre (bottom) GCaMP6f signal. e, Heatmaps of average GCaMP6f signal. f, Average GCaMP6f signal in during entries in the open arm (OA) or closed arm (CA). D1: n = 15 OA, n = 33 CA, D2: n = 18 OA, n = 32 CA. g, Signal intensity (AUC) by EPM compartment. LMM-ANOVA: compartment F2,20 = 58.21 p < 0.0001, cell type F1,10 = 1.12 p = 0.3142, compartment x cell type F2,20 = 1.04 p = 0.3724; followed by FDR-adjusted post-hoc tests. Data represented as mean ± sem. Scale bars 100 µm.
