Extended Data Fig. 7: Calcium imaging of vSub D1 and D2 neuronal activity during anxiety-related testing.
From: Dopamine D1–D2 signalling in hippocampus arbitrates approach and avoidance
a, Experimental schematic (n = 7 D1, n = 8 D2 male mice). b, Representative D1-Cre (top) and D2-Cre (bottom) GCaMP6s signal. c, Heatmaps of average GCaMP6s signal. d, Average GCaMP6s signal during entries in the arena center (D1: n = 50; D2: n = 149 entries). e, Average GCaMP6s signal around exits from immobility in arena corners (D1: n = 231; D2: n = 178 events). Dotted line indicates movement initiation. f, Signal intensity (AUC) by OFT zone. LMM-ANOVA: zone F3,39 = 6.40 p = 0.0012, cell type F1,13 = 0.33 p = 0.5742, zone x cell type F3,39 = 7.74 p = 0.0004; followed by FDR-adjusted post-hoc tests. g, Experimental schematic (n = 6 D1, n = 7 D2 male mice). h, Representative D1-Cre (top) and D2-Cre (bottom) GCaMP6s signal. The first (1) feeding bout is denoted. i, Heatmaps of average GCaMP6s signal. P food pellet. j, Average GCaMP6s signal around the initiation of feeding bouts (D1: n = 68; D2: n = 88 bouts). k, Average GCaMP6s signal around exits from immobility in arena corners (D1: n = 87; D2: n = 32 events). Dotted line indicates movement initiation. l, Signal intensity (AUC) by NSF arena zone and feeding activity in the center zone. LMM-ANOVA: zone F4,44 = 10.36 p < 0.0001, cell type F1,11 = 0.64 p = 0.4405, zone x cell type F4,44 = 1.65 p = 0.1785; followed by FDR-adjusted post-hoc tests. Data represented as mean ± sem.
