Extended Data Fig. 11: vSub dopamine, D1 and D2 correlates of approach and avoidance in PMA.
From: Dopamine D1–D2 signalling in hippocampus arbitrates approach and avoidance
a, Experimental schematic (n = 4 RdLight−1, n = 13 D1-GCaMP6s, n = 8 D2-GCaMP6s male mice). b, Average GCaMP6s signal around electric foot-shock onset when on the grid (left, RdLight-1 n = 22, D1-GCaMP6s n = 69, D2-GCaMP6s n = 45 events), reward magazine entry (middle, RdLight−1 n = 25, D1-GCaMP6s n = 6, D2-GCaMP6s n = 11 events) or the first platform exit after tone/shock (right, RdLight-1 n = 18, D1-GCaMP6s n = 43, D2-GCaMP6s n = 40 events). c, Quantification of peri-event signal changes. LMM-ANOVAs (Foot-shock, DA: F7,147 = 18.64 p < 0.0001. Foot-shock, D1: F7,475.06 = 79.37 p < 0.0001. Foot-shock, D2: F7,308 = 48.69 p < 0.0001. Reward, DA: F7,161 = 6.88 p < 0.0001. Reward, D1: F7,35 = 0.27 p = 0.9605. Reward, D2: F7,70 = 3.15 p = 0.0059. First platform exit, DA: F7,119 = 7.64 p < 0.0001. First platform exit, D1: F7,294 = 1.19 p = 0.3109. First platform exit, D2: F7,273 = 9.72 p < 0.0001); followed by FDR-adjusted post-hoc tests (all pairwise comparisons were computed but only the ones significant vs the first 1.5 s time-bin are represented, p < 0.05*, <0.01**, <0.001***). d, Times of peak photometry RdLight−1 and D2-GCaMP6s signals after foot-shock (left, LMM-ANOVA: sensor F1,22.94 = 4.99 p = 0.0355) and first platform exit (right, LMM-ANOVA: sensor F1,19.468 = 1.49 p = 0.2376). e, Correlation of RdLight-1 and D2-GCaMP6s signal (n = 1 dual-injected male mouse) around foot-shocks (left; Pearson’s r = −0.18 p < 0.0001), reward consumption (Pearson’s r = −0.37 p < 0.0001) and first platform exit after tone end (right; Pearson’s r = −0.19, p < 0.0001). Data represented as mean ± sem, regression lines with their 95% confidence intervals.
