Extended Data Fig. 12: Chemogenetic manipulation of vSub D1 and D2 neurons in the PMA task.
From: Dopamine D1–D2 signalling in hippocampus arbitrates approach and avoidance
a, Experimental schematic (n = 10 D1-mCherry, n = 7 D1-hM4Di male mice). b, Approach behavior (lever presses) during extinction sessions, during tone presentation (left; LMM-ANOVA: session F4,60 = 6.50 p = 0.0002, DREADD F1,15 = 0.47 p = 0.5023, session x DREADD F4,60 = 0.31 p = 0.8692) and inter-trial intervals (ITI, right; LMM-ANOVA: session F4,60 = 1.10 p = 0.3644, DREADD F1,15 = 1.45 p = 0.2466, session x DREADD F4,60 = 1.35 p = 0.2623). c, Avoidance behavior (time on platform) during extinction sessions. LMM-ANOVA: session F4,60 = 21.56, p < 0.0001, DREADD F1,15 = 0.003 p = 0.9568, session x DREADD F4,60 = 0.36 p = 0.8348. d, Freezing upon tone presentation during extinction sessions. LMM-ANOVA: session F4,60 = 2.92 p = 0.0283, DREADD F1,15 = 2.73 p = 0.1191, session x DREADD F4,60 = 0.19 p = 0.9424. e, Approach behavior (lever presses) during conditioning sessions for D1-Cre mice during tone presentation (left; LMM-ANOVA: session F9,135 = 3.05 p = 0.0024, DREADD F1,15 = 0.04 p = 0.8533, session x DREADD F9,135 = 0.27 p = 0.9808) or during inter-tone intervals (ITI; right; LMM-ANOVA: session F9,135 = 5.61 p < 0.0001, DREADD F1,15 = 0.003 p = 0.9608, session x DREADD F9,135 = 1.08 p = 0.3846). f, Avoidance (time on platform) behavior during conditioning sessions for D1-Cre mice. LMM-ANOVA: session F9,133.1 = 15.04 p < 0.0001, DREADD F1,15.006 = 2.79 p = 0.1156, session x DREADD F9,133.1 = 1.67 p = 0.1027. g, Experimental schematic (n = 11 D2-mCherry, n = 9 D2-hM4Di male mice). h, Approach behavior (lever presses) during extinction sessions, during tone presentation (left; LMM-ANOVA: session F4,72 = 64.75 p < 0.0001, DREADD F1,18 = 5.27 p = 0.0339, session x DREADD F4,72 = 0.83 p = 0.5099) and inter-trial intervals (ITI, right; LMM-ANOVA: session F4,72 = 22.15 p < 0.0001, DREADD F1,18 = 0.006 p = 0.9379, session x DREADD F4,72 = 2.03 p = 0.0994). i, Avoidance behavior (time on platform) during extinction sessions. LMM-ANOVA: session F4,72 = 26.69, p < 0.0001, DREADD F1,18 = 6.13 p = 0.0234, session x DREADD F4,72 = 0.57 p = 0.6858. j, Freezing upon tone presentation during extinction sessions. LMM-ANOVA: session F4,72 = 4.25 p = 0.0038, DREADD F1,18 = 0.003 p = 0.9570, session x DREADD F4,72 = 1.45 p = 0.2278. k, Approach behavior (lever presses) during conditioning sessions for D2-Cre mice during tone presentation (left; LMM-ANOVA: session F9,162 = 10.30 p < 0.0001, DREADD F1,18 = 1.03 p = 0.3227, session x DREADD F9,162 = 1.54 p = 0.1373) or during inter-tone intervals (ITI; right; LMM-ANOVA: session F9,162 = 5.50 p < 0.0001, DREADD F1,18 = 4.12 p = 0.0575, session x DREADD F9,162 = 1.11 p = 0.3589). l, Avoidance behavior (time on platform) during conditioning sessions for D2-Cre mice (LMM-ANOVA: session F9,162 = 26.31 p < 0.0001, DREADD F1,18 = 0.44 p = 0.5170, session x DREADD F9,162 = 1.91 p = 0.05385). Data represented as mean ± sem.
