Extended Data Fig. 5: The effect of metformin and CTPI2 on mitochondrial respiration in Dnmt3aR882H/+HSPCs.
From: Mitochondrial metabolism sustains DNMT3A-R882-mutant clonal haematopoiesis
(a) Oxygen consumption rate in Dnmt3aR882H/+ treated in vitro with 1.25 mM metformin for 2 h, mean ± SD. n = 11 for vehicle and n = 12 for metformin, indicating three biological replicates per condition, for metformin performed in 4 replicates and for vehicle, two performed in 3 and one in 4 replicates. One of three independent experiments is shown. (b) Basal, maximal respiration and ATP-production were compared between metformin and vehicle treatment, mean ± SD. P by two-sided t-test. Data were normalised to each vehicle control. n = 7 biological replicates per condition from three independent experiments. (c) Oxygen consumption rate in Dnmt3aR882H/+ treated with 150uM of CTPI2 for 2 h, mean ± SD. n = 11 for vehicle and n = 10 for CTPI2, indicating three biological replicates per condition, for CTPI2 performed in 4, 3 and 3 replicates and for vehicle, two performed in 3 and one in 4 replicates. One of two independent experiments is shown (d) Basal, maximal respiration and ATP-production were compared between CTPI2 and vehicle, mean ± SD, P by two-sided t-test. Data were normalised to each vehicle control. n = 4 biological replicates per condition from two independent experiments.
