Extended Data Fig. 9: Multi-ciliated cells at the transition between the corpus callosum and the alveus.
From: Light-microscopy-based connectomic reconstruction of mammalian brain tissue
a, Regional overview image. b, 3D rendering of a LICONN imaging volume (top, recorded with a 20× objective lens) of the region indicated by the box in the overview, highlighting multi-ciliated cells. The bright structures correspond to basal bodies. Bottom: Single imaging planes from the same volume as indicated by the magenta box in the upper panel. Intensity lookup tables in the individual panels are adjusted to either emphasize the intensely labelled basal bodies or the local cellular architecture. c, Left: Rendering of a LICONN imaging volume recorded with a high-NA objective lens in the same region from a different specimen. Right: Single imaging plane showing multi-ciliated cells and magnified view of cilia with intensity lookup table adjusted to visualize the axonemes of cilia. Representative of replicates in n = 2 mice.
