Extended Data Fig. 3: Synaptic labelling in mossy fibre boutons in the CA3 stratum lucidum.
From: Light-microscopy-based connectomic reconstruction of mammalian brain tissue
a, Mossy fibre boutons forming synapses with complex spines (thorny excrescences) at proximal dendrites of CA3 pyramidal neurons in hippocampus, interspersed with bundles of mossy fibres (non-myelinated axons of DG granule cells). Single plane of a LICONN imaging volume including the structural channel (grey) and immunolabelling for VGLUT1 (vesicular glutamate transporter 1, magenta), a synaptic vesicle marker in excitatory synapses, and the active-zone marker RIM1/2 (cyan). Bottom: Immunolabelling and structural channels displayed separately and as overlay for the two mossy fibre boutons and their postsynaptic partners in the box in the top panel. b, Similar measurement with immunolabelling for presynaptic bassoon and the postsynaptic scaffolding protein PSD95, present at excitatory synapses. Immunolabelling for RIM1/2 and VGLUT1 is representative of n = 2 technical replicates and bassoon/PSD95 of n = 3 replicates across n = 2 mice.
