Extended Data Fig. 8: The ileal immune phenotypes of Apol9a/b-deficient mice are associated with Bacteroidales.
From: Targeting symbionts by apolipoprotein L proteins modulates gut immunity
a, Schematic diagram of FMT of ABX-treated Apol9a/b+/+ or Apol9a/b–/– mice with ileal microbiota from a WT C57BL/6 strain largely devoid of gut Bacteroidales. After 4 weeks, indicated ileal tissues or cells were analyzed. b, 16S rRNA gene sequencing of the microbial composition of the ileal contents from a WT C57BL/6 strain largely devoid of gut Bacteroidales (n = 3). c-e, Q-PCR (c, ABX: n = 4, FMT: n = 6) and flow cytometry (d,e, ABX: n = 5, FMT: n = 6) analysis of representative MHC-II molecule expression in ileal ECs from mice as described in (a). f and g, Ileal intraepithelial CD4+CD8αα+ cells from mice as described in (a) were analyzed by flow cytometry. The representative flow cytometry plots (f) and CD4+CD8αα+ cell percentages (g, ABX: n = 5, FMT: n = 6) are shown. Data are representative from two independent experiments (a-g). n represents biologically independent samples (b) or biologically independent animals (c,e,g). Data are presented as mean ± SEM. Statistical analysis was performed using one-way ANOVA followed by Bonferroni post hoc test (c,e,g).
