Extended Data Fig. 9: Ileal immunomodulatory functions of APOL9 require Bacteroides-APOL9 interaction in vivo.
From: Targeting symbionts by apolipoprotein L proteins modulates gut immunity
a, Q-PCR analysis of the relative abundance of B. thetaiotaomicron in the feces from ABX-treated Apol9a/b+/+ or Apol9a/b–/– mice transferred with a gut Bacteroidales-minus ileal microbiota plus WT or BT_0871-deficient B. thetaiotaomicron strain (n = 8). b, ELISA analysis (n = 8) of ileal IFNγ production in the mice as described in (a). c-d, Q-PCR (c, n = 8), and flow cytometry (d, n = 8) analysis of representative MHC-II molecule expression in ileal ECs from mice as described in (a). e and f, Ileal intraepithelial CD4+CD8αα+ cells from mice as described in (a) were analyzed by flow cytometry. The representative flow cytometry plots (e) and CD4+CD8αα+ cell percentages (f, n = 8) are shown. Data are pooled from two independent experiments (a-f). n represents biologically independent animals (a-d,f). Data are presented as mean ± SEM. Statistical analysis was performed using one-way ANOVA followed by Bonferroni post hoc test (a-d,f).
