Extended Data Fig. 8: 13C-Glucose tracing in Cse Het and KO mice on Cys-deficient CR diet.
From: Unravelling cysteine-deficiency-associated rapid weight loss
a, Scheme of 13C glucose tracing experiment (n = 4 for liver, n = 6 for Het, n = 4 for KO for urine, all males). b, Pathway depicting the formation of heavy carbon labelled metabolites from m + 6 glucose. c, Levels of m + 3 lactate in the livers of Cse Het or KO mice at a time point of 45 min. d, Levels of m + 3 orotate in urine of Cse Het or KO mice at a time point of 2 h. e and f, Levels of guanidoacetate m + 2 in liver (e) at 45 min and urine (f) at 2 h in Het or KO mice. g and h, Levels of creatine m + 2 in liver (g) at 45 min and urine (h) at 2 h in Het or KO mice. i, Expression of Ckmt1 and Ckm in epididymal fat of Cse Het and KO mice on No Cys diet (see also Supplementary Table 4) (n = 3 for Het, n = 4 for KO). j, Peak energy expenditure (EE) at Day 3 of a No Cys diet normalized to body weight, subsequently normalized to Het data (n = 9 for Het n = 7 for KO). k, Creatine levels in liver at Day 6 on a No Cys diet in Het and KO mice (n = 4 for Het, n = 5 for KO). Panel (a) was created using BioRender.com. Unpaired t-test c-k, Data are mean ± s.e.m. *P < 0.05, ***P < 0.001 and ****P < 0.0001.
