Fig. 2: Cysteine deficiency drives rapid metabolic changes and loss of white adipose tissue mass.
From: Unravelling cysteine-deficiency-associated rapid weight loss
a–d Metabolic cage profiles of male Cse+/− and Cse−/− mice on CR (n = 5 (Cse+/−) and 3 (Cse−/−)), demonstrating the experimental design (food was given daily between 2 and 3 pm) (a), body-weight measurements (b), locomotor activity (c) and the RER (d). e, Body fat percentages, as determined by DEXA scans on day 7, of mice fed on no-Cys and control diets, either CR or ad libitum. n = 4 per group. NS, not significant. f–h, Representative images (n = 4 per group) of subcutaneous fat pads from Cse+/− or Cse−/− male mice on CR with Ctrl or no-Cys diets stained with haematoxylin and eosin (H&E) on days 3 and 7 of cysteine restriction (f), and by immunohistochemistry (IHC) staining of caspase 3 on day 7 of cysteine restriction (g) and of UCP1 on days 3 and 7 of cysteine restriction (h). The diagram in a was created using BioRender and image from https://www.tse-systems.com/. For d and e, statistical analysis was performed using multiple unpaired t-tests. Data are mean ± s.d. *P < 0.05. Scale bars, 100 μm (f and g) and 200 μm (h).
